Leroy Douresseaux compares the tone of the manga to Strawberry 100%, and feels that the manga is full of "comic violence that is brutal and bloody or innuendo defined by female ninja (kunoichi) in some state of undress."
Davey C. Jones enjoyed the "voluptuous" character designs of the girls.
Matthew Alexander found the character of the spirit tablet imbued with the protagonist's mother's spirit to be very funny, and found the protagonist to be a "likeable character" who "cares more about the feelings of those around him than he does about his own wellbeing".
Dan Polley felt the first volume had "potential", and that the secondary characters were intriguing.
Joseph Luster, writing for Otaku USA, felt that the manga was in an awkward position: "too fan-servicey for some, and not enough for others".
Ninja Girls volume 1 debuted sixth on the New York Times manga bestseller list for the week of August 23-29, 2009. The following week, Ninja Girls volume 1 slipped to eighth position.