- Kuro Takagi - The hero of this horror manga, he's afraid of the dark. His mother tells him of his father's school on her deathbed, leading Kuro to an academy that teaches folklore and dark arts history in place of math and other academics. Later on he gains a spirit creature named Pochi.
- "Lady" Mao Toyama - Kuro's (unbeknownst to him) long lost sister. Being highly spiritually aware, she is able to summon a butterfly-alien familiar named "Neferti," and can transform her into a sword. Craves attention from her father, who has decided to focus on her brother Kuro instead. She is thus filled with contempt for Kuro.
- Kuro and Mao's father - The unnamed headmaster of the school, he gave his son a ring which will allow him to strengthen his powers, yet also attract Succubi and Nosferatu. He created the school in an effort to strengthen the remnants of the monster species that was nearly wiped out two decades ago by "Carriers." Apparently wants his son dead, and treats his daughter more like a pet than family.
- Miki Akasaka - A girl with a fiery personality and friend of Kana Okamoto. She is willing to do anything to have her friend return to her normal human state.
- Kana Okamoto - The first character to die in the series, she fell in love with Mr Tsuchiya the biology teacher, and ended up becoming a zombie after her corpse was implanted with "evil genes."
- Louie Nishiwaki - A smart and strong boy born to a Japanese father and African-American mother, he was often excluded from group activities in his youth due to his dark skin. He's also afraid of blood, and faints at first site of it.
- Akimi Shimizu - A girl with light-colored hair and upbeat personality, she is madly in love with Mr. Tsuchiya, a confirmed "nosferatu." Her love for him continues even after she found out about her teacher and his experiment on Kana Okamoto.
- Ise Tetsuzo - A dark-skinned boy with a twin sister in the hospital, he is willing to become a Nosferatu in order to discover the secret of immortality. He is dating a middle school girl and plays in a rock band. He's the series resident "bad boy."
- Mr. Tsuchiya / John Garous - The school's biology teacher, and secretly the last remaining member of the Nosferatu (monsters that, in Dark Edge, look like classical depictions of the devil). Kana Okamoto fell madly in love with him, so Garous turned her into a zombie. Currently the manga's main antagonist.
- Sonobe - A new teacher, and member of the Succubus race. Has a youthful appearance and is obsessed with drinking Kuro's blood.
- "Carriers" - Humans with magical powers, these Carriers were the ones responsible for bringing the Nosferatu race to the brink of extinction back in the 1980s.
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